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Original researchPeer-reviewedOpen Access · CC BY-NC-SA 4.0

Comparison of the Toxogen® rapid test with ELISA immunoenzymatic technique for toxoplasmosis serology in pregnant women

Francine Isabelle Rakotonindrina1, Rakotomalala Rivo S2, Tsatoromila Fenosoa A3, Rabenandrianina Tahirimalala4, Rajaonatahina Davidra5, Rakotovao Luc A6, Rakoto Alson Olivat A7, Rasamindrakotroka Andry8, Randriamanantany Zely9, Razanakolona Lala R S10

  1. 1 Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar. (corresponding author)
  2. 2 Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar.
  3. 3 Directorate of Blood Transfusion - Faculty of Medicine, University of Antananarivo.
  4. 4 Laboratory of the Professor Zafisaona Gabriel (PZaGa) University Hospital Center- Faculty of Medicine, University of Mahajanga, Madagascar.
  5. 5 Paraclinical Training and Research Unit in Immunology - Faculty of Medicine, University of Antananarivo.
  6. 6 CHU / JRB Laboratory - Faculty of Medicine, University of Antananarivo.
  7. 7 CHU / JRB Laboratory - Faculty of Medicine, University of Antananarivo.
  8. 8 Laboratory for Training and Research in Medical Biology, Faculty of Medicine, University of Antananarivo.
  9. 9 Directorate of Blood Transfusion - Faculty of Medicine, University of Antananarivo.
  10. 10 National Reference Laboratory for HIV / AIDS and Sexually Transmitted Infections - Ministry of Public Health - Faculty of Medicine, University of Antananarivo.

DOI: 10.5281/zenodo.3931160 · Published 2020-05-28 · pp. 1-6

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Abstract

The aim of this study was to compare the technical performance of the Toxogen® rapid test against the ELISA technique for serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Methods and material: A prospective, descriptive and analytical study was conducted over a period of 7 months from January to July 2018. One hundred and eighty-two toxoplasmosis serologies were carried out on 144 pregnant women followed in two hospitals in Mahajanga and in a private laboratory in Antananarivo. Two serologies were performed for each case, the first by the rapid test Toxogen®, a latex agglutination test, and the second by the IgG-ELISA and IgM-ELISA technique.

Results: The Toxogen® rapid test had a sensitivity of 62.5%, a specificity of 97.1%, a Positive Predictive Value of 94.3%, a Negative Predictive Value of 76.7% compared to the IgG-ELISA technique. The correlation between the dilution titer by the Toxogen® rapid test and the optical density (OD) of anti-toxoplasmics IgG by the ELISA technique was very low (r² = 0.17, p <0.001). The crude prevalence of toxoplasmosis was 30,6% (n=44) by the Toxogen® rapid test and 46.5% (n=67) by the IgG-ELISA technique, the difference was significant (p <0.001). We had 2 cases (0.01%) positives by the IgM-ELISA which were not detected by the Toxogen® rapid test.

Conclusion: The Toxogen® rapid test cannot substitute the ELISA technique for the serological screening and/or serological monitoring of toxoplasmosis in pregnant women.

Keywords: ELISA; pregnant women; latex agglutination test; serology; toxoplasmosis.

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